Adhesion molecules from the diatom Phaeodactylum tricornutum (Bacillariophyceae): genomic identification by amino‐acid profiling and in vivo analysis
Identifieur interne : 000186 ( Istex/Checkpoint ); précédent : 000185; suivant : 000187Adhesion molecules from the diatom Phaeodactylum tricornutum (Bacillariophyceae): genomic identification by amino‐acid profiling and in vivo analysis
Auteurs : Anusuya Willis [Australie, France] ; Maeve Eason-Hubbard [Australie, Royaume-Uni] ; Oliver Hodson [Australie] ; Uma Maheswari [France, Royaume-Uni] ; Chris Bowler [France] ; Richard Wetherbee [Australie, France]Source :
- Journal of Phycology [ 0022-3646 ] ; 2014-10.
Abstract
Cell adhesion molecules (CAMs) are important in prokaryotes and eukaryotes for cell–cell and cell–substratum interactions. The characteristics of adhesive proteins in the model diatom Phaeodactylum tricornutum were investigated by bioinformatic analysis and in vivo characterization. Bioinformatic analysis of the protein coding potential of the P. tricornutum genome used an amino‐acid profile that we developed as a new system to identify uncharacterized or novel CAMs. Putative diatom CAMs were identified and seven were characterized in vivo, by generation of transgenic diatom lines overexpressing genes encoding C‐terminal yellow fluorescent protein (YFP) fusion proteins. Three of these selected genes encode proteins with weak similarity to characterized proteins, a c‐type lectin and two fasciclins, whereas the others are novel. The resultant cell lines were investigated for alterations in their adhesive ability. Whole cell‐substratum adhesion strength was measured in a fully turbulent flow chamber, while atomic force microscopy was used to quantify the relative frequency of adhesion, as well as the length and strength of single molecules in the secreted mucilage. Finally, quartz crystal microbalance analysis characterized the visco‐elastic properties and interaction of the mucilage–substratum interface. These combined studies revealed a range of phenotypes affecting adhesion, and led to the identification of candidate proteins involved in diatom adhesion. In summary, our study has for the first time combined bioinformatics and molecular physiological studies to provide new insights into diatom adhesive molecules.
Url:
DOI: 10.1111/jpy.12214
Affiliations:
- Australie, France, Royaume-Uni
- Angleterre, Oxfordshire, Victoria (État), Île-de-France
- Melbourne, Oxford, Paris
- Université d'Oxford, Université de Melbourne
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:480C2234880E2E5E99C211D18DAF5EB1159BB2E9Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Adhesion molecules from the diatom Phaeodactylum tricornutum (Bacillariophyceae): genomic identification by amino‐acid profiling and in vivo analysis</title>
<author><name sortKey="Willis, Anusuya" sort="Willis, Anusuya" uniqKey="Willis A" first="Anusuya" last="Willis">Anusuya Willis</name>
</author>
<author><name sortKey="Eason Ubbard, Maeve" sort="Eason Ubbard, Maeve" uniqKey="Eason Ubbard M" first="Maeve" last="Eason-Hubbard">Maeve Eason-Hubbard</name>
</author>
<author><name sortKey="Hodson, Oliver" sort="Hodson, Oliver" uniqKey="Hodson O" first="Oliver" last="Hodson">Oliver Hodson</name>
</author>
<author><name sortKey="Maheswari, Uma" sort="Maheswari, Uma" uniqKey="Maheswari U" first="Uma" last="Maheswari">Uma Maheswari</name>
</author>
<author><name sortKey="Bowler, Chris" sort="Bowler, Chris" uniqKey="Bowler C" first="Chris" last="Bowler">Chris Bowler</name>
</author>
<author><name sortKey="Wetherbee, Richard" sort="Wetherbee, Richard" uniqKey="Wetherbee R" first="Richard" last="Wetherbee">Richard Wetherbee</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:480C2234880E2E5E99C211D18DAF5EB1159BB2E9</idno>
<date when="2014" year="2014">2014</date>
<idno type="doi">10.1111/jpy.12214</idno>
<idno type="url">https://api.istex.fr/document/480C2234880E2E5E99C211D18DAF5EB1159BB2E9/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000D54</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000D54</idno>
<idno type="wicri:Area/Istex/Curation">000D54</idno>
<idno type="wicri:Area/Istex/Checkpoint">000186</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000186</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Adhesion molecules from the diatom <hi rend="italic">Phaeodactylum tricornutum</hi>
(Bacillariophyceae): genomic identification by amino‐acid profiling and in vivo analysis</title>
<author><name sortKey="Willis, Anusuya" sort="Willis, Anusuya" uniqKey="Willis A" first="Anusuya" last="Willis">Anusuya Willis</name>
<affiliation wicri:level="4"><country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Botany, The University of Melbourne, 3010, Parkville, Victoria</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Environmental and Evolutionary Genomics Section, CNRS UMR8197 INSERM U1024, Institut de Biologie de l'Ecole Normale Supérieure, 46 rue d'Ulm 75230, Paris Cedex 05</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Australie</country>
<wicri:regionArea>Current Address: Australian Rivers Institute, Griffith University, 170 Kessels Rd, Queensland, 4111, Nathan</wicri:regionArea>
<wicri:noRegion>Nathan</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Eason Ubbard, Maeve" sort="Eason Ubbard, Maeve" uniqKey="Eason Ubbard M" first="Maeve" last="Eason-Hubbard">Maeve Eason-Hubbard</name>
<affiliation wicri:level="4"><country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Botany, The University of Melbourne, Victoria, 3010, Parkville</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Current Address: Department of Earth Sciences, University of Oxford, Oxford</wicri:regionArea>
<placeName><settlement type="city">Oxford</settlement>
<region type="country">Angleterre</region>
<region type="comté" nuts="2">Oxfordshire</region>
<settlement type="city">Oxford</settlement>
</placeName>
<orgName type="university">Université d'Oxford</orgName>
</affiliation>
</author>
<author><name sortKey="Hodson, Oliver" sort="Hodson, Oliver" uniqKey="Hodson O" first="Oliver" last="Hodson">Oliver Hodson</name>
<affiliation wicri:level="4"><country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Botany, The University of Melbourne, Victoria, 3010, Parkville</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Maheswari, Uma" sort="Maheswari, Uma" uniqKey="Maheswari U" first="Uma" last="Maheswari">Uma Maheswari</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Environmental and Evolutionary Genomics Section, CNRS UMR8197 INSERM U1024, Institut de Biologie de l'Ecole Normale Supérieure, 46 rue d'Ulm 75230, Paris Cedex 05</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Current Address: EMBL Outstation ‐ Hinxton, European Bioinformatics Institute, CB10 1SD, Cambridge</wicri:regionArea>
<wicri:noRegion>Cambridge</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bowler, Chris" sort="Bowler, Chris" uniqKey="Bowler C" first="Chris" last="Bowler">Chris Bowler</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Environmental and Evolutionary Genomics Section, CNRS UMR8197 INSERM U1024, Institut de Biologie de l'Ecole Normale Supérieure, 46 rue d'Ulm 75230, Paris Cedex 05</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">France</country>
</affiliation>
</author>
<author><name sortKey="Wetherbee, Richard" sort="Wetherbee, Richard" uniqKey="Wetherbee R" first="Richard" last="Wetherbee">Richard Wetherbee</name>
<affiliation wicri:level="4"><country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Botany, The University of Melbourne, Victoria, 3010, Parkville</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">France</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Journal of Phycology</title>
<title level="j" type="alt">JOURNAL OF PHYCOLOGY</title>
<idno type="ISSN">0022-3646</idno>
<idno type="eISSN">1529-8817</idno>
<imprint><biblScope unit="vol">50</biblScope>
<biblScope unit="issue">5</biblScope>
<biblScope unit="page" from="837">837</biblScope>
<biblScope unit="page" to="849">849</biblScope>
<biblScope unit="page-count">13</biblScope>
<date type="published" when="2014-10">2014-10</date>
</imprint>
<idno type="ISSN">0022-3646</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-3646</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Cell adhesion molecules (CAMs) are important in prokaryotes and eukaryotes for cell–cell and cell–substratum interactions. The characteristics of adhesive proteins in the model diatom Phaeodactylum tricornutum were investigated by bioinformatic analysis and in vivo characterization. Bioinformatic analysis of the protein coding potential of the P. tricornutum genome used an amino‐acid profile that we developed as a new system to identify uncharacterized or novel CAMs. Putative diatom CAMs were identified and seven were characterized in vivo, by generation of transgenic diatom lines overexpressing genes encoding C‐terminal yellow fluorescent protein (YFP) fusion proteins. Three of these selected genes encode proteins with weak similarity to characterized proteins, a c‐type lectin and two fasciclins, whereas the others are novel. The resultant cell lines were investigated for alterations in their adhesive ability. Whole cell‐substratum adhesion strength was measured in a fully turbulent flow chamber, while atomic force microscopy was used to quantify the relative frequency of adhesion, as well as the length and strength of single molecules in the secreted mucilage. Finally, quartz crystal microbalance analysis characterized the visco‐elastic properties and interaction of the mucilage–substratum interface. These combined studies revealed a range of phenotypes affecting adhesion, and led to the identification of candidate proteins involved in diatom adhesion. In summary, our study has for the first time combined bioinformatics and molecular physiological studies to provide new insights into diatom adhesive molecules.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Oxfordshire</li>
<li>Victoria (État)</li>
<li>Île-de-France</li>
</region>
<settlement><li>Melbourne</li>
<li>Oxford</li>
<li>Paris</li>
</settlement>
<orgName><li>Université d'Oxford</li>
<li>Université de Melbourne</li>
</orgName>
</list>
<tree><country name="Australie"><region name="Victoria (État)"><name sortKey="Willis, Anusuya" sort="Willis, Anusuya" uniqKey="Willis A" first="Anusuya" last="Willis">Anusuya Willis</name>
</region>
<name sortKey="Eason Ubbard, Maeve" sort="Eason Ubbard, Maeve" uniqKey="Eason Ubbard M" first="Maeve" last="Eason-Hubbard">Maeve Eason-Hubbard</name>
<name sortKey="Hodson, Oliver" sort="Hodson, Oliver" uniqKey="Hodson O" first="Oliver" last="Hodson">Oliver Hodson</name>
<name sortKey="Wetherbee, Richard" sort="Wetherbee, Richard" uniqKey="Wetherbee R" first="Richard" last="Wetherbee">Richard Wetherbee</name>
<name sortKey="Willis, Anusuya" sort="Willis, Anusuya" uniqKey="Willis A" first="Anusuya" last="Willis">Anusuya Willis</name>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Willis, Anusuya" sort="Willis, Anusuya" uniqKey="Willis A" first="Anusuya" last="Willis">Anusuya Willis</name>
</region>
<name sortKey="Bowler, Chris" sort="Bowler, Chris" uniqKey="Bowler C" first="Chris" last="Bowler">Chris Bowler</name>
<name sortKey="Bowler, Chris" sort="Bowler, Chris" uniqKey="Bowler C" first="Chris" last="Bowler">Chris Bowler</name>
<name sortKey="Maheswari, Uma" sort="Maheswari, Uma" uniqKey="Maheswari U" first="Uma" last="Maheswari">Uma Maheswari</name>
<name sortKey="Wetherbee, Richard" sort="Wetherbee, Richard" uniqKey="Wetherbee R" first="Richard" last="Wetherbee">Richard Wetherbee</name>
</country>
<country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Eason Ubbard, Maeve" sort="Eason Ubbard, Maeve" uniqKey="Eason Ubbard M" first="Maeve" last="Eason-Hubbard">Maeve Eason-Hubbard</name>
</region>
<name sortKey="Maheswari, Uma" sort="Maheswari, Uma" uniqKey="Maheswari U" first="Uma" last="Maheswari">Uma Maheswari</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000186 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000186 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Istex |étape= Checkpoint |type= RBID |clé= ISTEX:480C2234880E2E5E99C211D18DAF5EB1159BB2E9 |texte= Adhesion molecules from the diatom Phaeodactylum tricornutum (Bacillariophyceae): genomic identification by amino‐acid profiling and in vivo analysis }}
This area was generated with Dilib version V0.6.33. |